flA

Enzyme Information
reference: 10.1016/S0014-5793(03)00688-4
test: incubation of 0.1 ml protein, 0.2 mM SAM and 5.0 mM KF in 50 mM Tris-HCl buffer (pH=7.8), followed by HPLC analysis
producer: Streptomyces cattleya NRRL 8057
phmm-model: SAM_fluorinase.hmm

Gene Data
phmm effect mutation gene_name reference
80 F location Thr80 flA DOI: 10.1038/nature02280
271 H-bond network with 5'-FDA and methionine bound R270 flA DOI: 10.1038/nature02280
269 H-bond network with 5'-FDA and methionine bound S269 flA DOI: 10.1038/nature02280
214 neighbouring residue of the H-bond network with 5'-FDA and methionine F213 flA DOI: 10.1038/nature02280
216 H-bond network with 5'-FDA and methionine bound N215 flA DOI: 10.1038/nature02280
255 neighbouring residue of the H-bond network with 5'-FDA and methionine F254 flA DOI: 10.1038/nature02280
280 H-bond network with 5'-FDA and methionine bound A279 flA DOI: 10.1038/nature02280
50 H-bond network with 5'-FDA and methionine bound W50 flA DOI: 10.1038/nature02280
23 H-bond network with 5'-FDA and methionine bound S23 flA DOI: 10.1038/nature02280
21 H-bond network with 5'-FDA and methionine bound D21 flA DOI: 10.1038/nature02280
158 None S158 flA DOI: 10.1021/ja0731569
- reduced activity T80A flA DOI: 10.1021/ja0731569
- reduced activity T80S flA DOI: 10.1021/ja0731569
- reduced activity S158A flA DOI: 10.1021/ja0731569
- highly reduced activity F156A flA DOI: 10.1021/ja0731569
- highly reduced activity F156V flA DOI: 10.1021/ja0731569
- no activity D16S flA DOI: 10.1021/ja0731569
- no activity D16A flA DOI: 10.1021/ja0731569
- no activity D16N flA DOI: 10.1021/ja0731569
- reduced activity S158G flA DOI: 10.1021/ja0731569
- F location F156-S158 flA DOI: 10.1038/nature02280
211 neighbouring residue of the H-bond network with 5'-FDA and methionine D210 flA DOI: 10.1038/nature02280
16 H-bond network with 5'-FDA and methionine bound; 3′-OH of 2'd-FDA accomodates the H-bond with the O1 and O2 of D16 D16 flA DOI: 10.1038/nature02280; 10.1039/b600574h
None hydrogen bond with Cl or F; larger distance with Cl S156? flA DOI: P DOI: M DOI: C DOI: I DOI: D DOI: : DOI: DOI: P DOI: M DOI: C DOI: 3 DOI: 3 DOI: 1 DOI: 4 DOI: 1 DOI: 9 DOI: 5
77 F location; 3′-OH of 2′d-FDA bonds with the NH of Y77; H-bond network with 5'-FDA and methionine bound Y77 flA DOI: 10.1038/nature02280; 10.1039/b600574h
156 H-bond network with 5'-FDA and methionine bound F156 flA DOI: 10.1038/nature02280