SaDAH

Enzyme Information

reference: 10.1038/s41467-020-15777-w
host: E. coli BL21 (DE3)
test: 25 mM Tris buffer, pH = 8.0, enzyme (20 µM), (−)-dechloroacutumine (100 µM), 2OG (500 µM), NaCl (1 mM), sodium ascorbate (5 mM), and (NH4)2Fe(So4)2 (2 mM) and LC-HRAM-MS
producer: Menispermaceae

Gene Data

effect	mutation	gene_name	reference
exclusive hydroxylase activity on (−)-dechloroacutumine and gives 11-hydroxy-dechloroacutumine	G226D	SaDAH	DOI: 10.1038/s41467-020-15777-w