PrnA
Enzyme Information
Gene Data
| phmm | effect | mutation | gene_name | reference |
|---|---|---|---|---|
| 444 | active site | Y444 | PrnA | DOI: 10.1002/anie.201007896 |
| 443 | active site | Y443 | PrnA | DOI: 10.1002/anie.201007896 |
| 450 | active site | E450 | PrnA | DOI: 10.1002/anie.201007896 |
| 454 | active site | F454 | PrnA | DOI: 10.1002/anie.201007896 |
| 455 | active site; shields the indole ring | W455 | PrnA | DOI: 10.1002/anie.201007896 |
| 348 | active site | S347 | PrnA | DOI: 10.1002/anie.201007896 |
| 347 | active site | E346 | PrnA | DOI: 10.1002/anie.201007896 |
| 101 | active site; shields the indole ring | H101 | PrnA | DOI: 10.1002/anie.201007896 |
| 79 | active site | K79 | PrnA | DOI: 10.1002/anie.201007896 |
| 103 | active site; shields the indole ring | F103 | PrnA | DOI: 10.1002/anie.201007896 |
| - | decreased kcat , increased Km | H101A | PrnA | DOI: 10.1002/anie.201007896 |
| - | decreased kcat , increased Km | W455A | PrnA | DOI: 10.1002/anie.201007896 |
| - | increased Km, decreased kcat compared to WT | F103A | PrnA | DOI: 10.1002/anie.201007896 |
| - | inactive | F103A/W455A | PrnA | DOI: 10.1002/anie.201007896 |
| - | inactive | E346D | PrnA | DOI: 10.1002/anie.201007896 |
| - | inactive | K79A | PrnA | DOI: 10.1002/anie.201007896 |
| - | reduced activity | E346Q | PrnA | DOI: 10.1002/anie.201007896 |
| - | reduced Km, slightly increased Vmax | W272F | PrnA | DOI: 10.1002/anie.200802466 |
| - | increased Km, slightly increased Vmax | W274F | PrnA | DOI: 10.1002/anie.200802466 |
| - | almost the same Km as the WT, double Vmax | W272F/W274F | PrnA | DOI: 10.1002/anie.200802466 |
| - | no significant change in Km and Vmax | W272A | PrnA | DOI: 10.1002/anie.200802466 |
| - | inactive | W274A | PrnA | DOI: 10.1002/anie.200802466 |
| - | decreased Km, decrease Vmax | S347A | PrnA | DOI: 10.1002/anie.200802466 |
| - | couldn't be expressed/purified? | W272A/W274A | PrnA | DOI: 10.1002/anie.200802466 |