PltA

Enzyme Information
reference: 10.1016/j.jsb.2015.09.013
host: Escherichia coli BL21 (DE3)
test: Crystallization
producer: Pseudomonas fluorescens Pf-5
phmm-model: FDH_pyrrole.hmm

Gene Data
phmm effect mutation gene_name reference
130 dimeric interface V141 PltA DOI: 10.1016/j.jsb.2015.09.013
291 FAD binding; isoalloxazine ring Tyr299 PltA DOI: 10.1016/j.jsb.2015.09.013
35 None Glu35 PltA DOI: 10.1016/j.jsb.2015.09.013
- hairpin between beta-bet1 strands and helix alpha1; FAD binding GxGxxG PltA DOI: 10.1016/j.jsb.2015.09.013
270 chloride binding; NOT conserved in other FDHs due to lack of bound chloride at analogous site Glu278 PltA DOI: 10.1016/j.jsb.2015.09.013
271 chloride binding; NOT conserved in other FDHs due to lack of bound chloride at analogous site N279 PltA DOI: 10.1016/j.jsb.2015.09.013
269 chloride binding; NOT conserved in other FDHs due to lack of bound chloride at analogous site R277 PltA DOI: 10.1016/j.jsb.2015.09.013
363 chloride binding; conserved in FDHs W371 PltA DOI: 10.1016/j.jsb.2015.09.013
321 FAD binding; oxyanion hole like environment Val329 PltA DOI: 10.1016/j.jsb.2015.09.013
288 chlorination mechanism; conserved in Trp-halogenases, but not in homologs; homologs have F326 in this position; FAD binding; isoalloxazine ring Glu296 PltA DOI: 10.1016/j.jsb.2015.09.013
230 FAD binding; isoalloxazine ring Trp239 PltA DOI: 10.1016/j.jsb.2015.09.013
194 FAD binding; isoalloxazine ring Ala203 PltA DOI: 10.1016/j.jsb.2015.09.013
318 FAD binding; isoalloxazine ring Ser326 PltA DOI: 10.1016/j.jsb.2015.09.013
315 FAD binding; isoalloxazine ring; chloride binding Pro323 PltA DOI: 10.1016/j.jsb.2015.09.013
312 FAD binding; isoalloxazine ring; chloride binding F320 PltA DOI: 10.1016/j.jsb.2015.09.013
44 FAD binding; isoalloxazine ring Val44 PltA DOI: 10.1016/j.jsb.2015.09.013
45 FAD binding; isoalloxazine ring Gly45 PltA DOI: 10.1016/j.jsb.2015.09.013
135 dimeric interface E146 PltA DOI: 10.1016/j.jsb.2015.09.013
160 nonpolar interactions between two symmetric patches of hydrophobic surface Ile171 PltA DOI: 10.1016/j.jsb.2015.09.013
158 nonpolar interactions between two symmetric patches of hydrophobic surface Val169 PltA DOI: 10.1016/j.jsb.2015.09.013
153 nonpolar interactions between two symmetric patches of hydrophobic surface Leu164 PltA DOI: 10.1016/j.jsb.2015.09.013
134 nonpolar interactions between two symmetric patches of hydrophobic surface Val145 PltA DOI: 10.1016/j.jsb.2015.09.013
131 nonpolar interactions between two symmetric patches of hydrophobic surface Phe142 PltA DOI: 10.1016/j.jsb.2015.09.013
121 H-bond with Glu40 main chain NH Glu132 PltA DOI: 10.1016/j.jsb.2015.09.013
26 H-bond with Glu132 side chain carboxyl group Glu40 PltA DOI: 10.1016/j.jsb.2015.09.013
156 nonpolar interactions between two symmetric patches of hydrophobic surface Arg167 PltA DOI: 10.1016/j.jsb.2015.09.013
- polar-charged residue contact with Arg167 Asp5 PltA DOI: 10.1016/j.jsb.2015.09.013
30 F39 side chain orientation; holding the diphosphate group; conserved in non-Trp halogenases; in a loop conserved in CmlS, CndH and 3NIX R41 PltA DOI: 10.1016/j.jsb.2015.09.013
32 holding the diphosphate group; conserved in non-Trp halogenases; in a loop conserved in CmlS, CndH and 3NIX H43 PltA DOI: 10.1016/j.jsb.2015.09.013
28 FAD binding F39 PltA DOI: 10.1016/j.jsb.2015.09.013
29 F39 side chain orientation Glu40 PltA DOI: 10.1016/j.jsb.2015.09.013
329 Predicted to interact with the phospho group of the substrate R337 PltA DOI: 10.1016/j.jsb.2015.09.013
337 chlorination mechanism; conserved in Trp-halogenases, but not in homologs; homologs have F326 in this position Glu345 PltA DOI: 10.1016/j.jsb.2015.09.013
62 conserved in FDHS; HOCl diffuses to this residue; Lys73 in PltA, Lys79 in PrnA and RebH, Lys71 in CmlS, Lys76 in CndH Lys73 PltA DOI: 10.1016/j.jsb.2015.09.013
112 holding the diphosphate group; conserved in non-Trp halogenases R123 PltA DOI: 10.1016/j.jsb.2015.09.013
117 polar-charged residue contact with Glu140 Arg128 PltA DOI: 10.1016/j.jsb.2015.09.013
132 main chain carbonyl oxygen is hydrogen bonded to the main chain nitrogen of the same residue of the other monomer and vica versa Gln143 PltA DOI: 10.1016/j.jsb.2015.09.013