PkHalD

Enzyme Information

reference: 10.1038/s41589-019-0355-x
host: E. coli BL21 Star (DE3)
test: ATP (2.5 mM), MgCl2 (5 mM), phosphoenolpyruvate (1 mM), NADH (0.3 mM), lactate dehydrogenase (10 U ml−1), pyruvate kinase (10 U ml−1), succinyl-CoA synthetase (3.2 U ml−1), coenzyme A (1 mM), sodium αKG (1 mM), (NH4)2Fe(SO4)2·6H2O (0.2 mM), sodium chloride (10 mM) and sodium ascorbate (2 mM) in 100 mM HEPES buffer (pH 7.5), 5 µM PkHalD
producer: Pseudomonas kilonensis

Gene Data

No available data