NapH1

Enzyme Information
reference: 10.1021/ja201088k
host: E. coli
test: MCD assay
producer: Streptomyces sp. CNQ-525
phmm-model: VHPO_VCPO_selective

Gene Data
phmm effect mutation gene_name reference
- inactive H420F NapH1 DOI: 10.1021/ja201088k
- exclusively catalyzes the formation of SF2415B1-based bromohydrins S427H NapH1 DOI: 10.1021/ja201088k
- cannot convert SF2415B1 to SF2415B3 (lost chlorination activity and hypobromite formation) K372F NapH1 DOI: 10.1021/acs.biochem.2c00338
- active for hypobromite formation; cannot catalyze chloro/bromocyclization of naphtomevalin K324M NapH1 DOI: 10.1021/acs.biochem.2c00338
- active for hypobromite formation K324R NapH1 DOI: 10.1021/acs.biochem.2c00338
- diminished hypobromite formation activity S427A NapH1 DOI: 10.1021/acs.biochem.2c00338
- cannot convert SF2415B1 to SF2415B3 (lost chlorination activity and hypobromite formation) R379V NapH1 DOI: 10.1021/acs.biochem.2c00338
- cannot convert SF2415B1 to SF2415B3 (lost chlorination activity and hypobromite formation) W369E NapH1 DOI: 10.1021/acs.biochem.2c00338
295 conserved in enantioselective halogenating enzymes; H in other structurally characterized VHPOs; bonds with vanadate and K324 S427 NapH1 DOI: 10.1021/acs.biochem.2c00338
362 vanadate cofactor coordination H494 NapH1 DOI: 10.1074/jbc.M611046200
246 charge-interaction with vanadate cofactor coordination R379 NapH1 DOI: 10.1021/acs.biochem.2c00338
191 extends towards the vanadate cofactor and S427; essential for stereospecific chlorofunctionalization; conserved in enantioselective halogenating enzymes K324 NapH1 DOI: 10.1021/acs.biochem.2c00338
239 charge-interaction with vanadate cofactor coordination K372 NapH1 DOI: 10.1021/acs.biochem.2c00338
356 charge-interaction with vanadate cofactor coordination R488 NapH1 DOI: 10.1021/acs.biochem.2c00338