FasV
Enzyme Information
Gene Data
| phmm | effect | mutation | gene_name | reference |
|---|---|---|---|---|
| - | weak halogenation activity | L309E | FasV | DOI: 10.1002/anie.202418843 |
| - | exclusively catalyses selective monohalogenation | I212T | FasV | DOI: 10.1002/anie.202418843 |
| - | significant decrease in halogenation activity | S310A | FasV | DOI: 10.1002/anie.202418843 |
| - | significant decrease in halogenation activity | S310D | FasV | DOI: 10.1002/anie.202418843 |
| - | significant decrease in halogenation activity | S310T | FasV | DOI: 10.1002/anie.202418843 |
| 29 | directly acts as a base to activate the hydroxy group at the ortho position of the halogenation site; dual activation mechanism | E49 | FasV | DOI: 10.1002/anie.202418843 |
| - | reduction in halogenation activity | E49A | FasV | DOI: 10.1002/anie.202418843 |
| - | reduction in halogenation activity | E49D | FasV | DOI: 10.1002/anie.202418843 |
| - | reduction in halogenation activity | E49Q | FasV | DOI: 10.1002/anie.202418843 |
| - | complete loss of activity | S50A | FasV | DOI: 10.1002/anie.202418843 |
| - | complete loss of activity | S50V | FasV | DOI: 10.1002/anie.202418843 |
| - | complete loss of activity | S50E | FasV | DOI: 10.1002/anie.202418843 |
| - | accumulation of dichlorinated compound (11) | F93W | FasV | DOI: 10.1002/anie.202418843 |
| - | lost polychlorination activity | L81A | FasV | DOI: 10.1002/anie.202418843 |
| - | similar regioselectivity with compound 1 and 3 | L81Y | FasV | DOI: 10.1002/anie.202418843 |
| - | when incubated with 1 or 3, there was a higher production of intermediate 5 and abolishment of 11; stricter regioselectivity and reduces the diversion toward other halogenation routes | T53A | FasV | DOI: 10.1002/anie.202418843 |
| - | accumulated more compound 2 and a monochlorinated compound | I212L | FasV | DOI: 10.1002/anie.202418843 |
| - | no polychlorination activity | I212T | FasV | DOI: 10.1002/anie.202418843 |
| 295 | H-network around substrate; likely hydrophobic pocket formation | L309 | FasV | DOI: 10.1002/anie.202418843 |
| 198 | H-network around substrate; binding of monochlorinated compound and subsequent polychlorination | I212 | FasV | DOI: 10.1002/anie.202418843 |
| - | conserved in homologous proteins; 212 is partially conserved featuring predominantly Ile and Thr residues | G209-D231 | FasV | DOI: 10.1002/anie.202418843 |
| 134 | substrate binding; dual activation mechanism | S150 | FasV | DOI: 10.1002/anie.202418843 |
| 77 | H-network around substrate; crucial for substrate binding | F93 | FasV | DOI: 10.1002/anie.202418843 |
| 33 | crucial for substrate binding | T53 | FasV | DOI: 10.1002/anie.202418843 |
| 30 | might promote the electrophilic addition of chloride to the substrate; catalytic activity | S50 | FasV | DOI: 10.1002/anie.202418843 |
| 77 | might be responsible for regioselectivity by regulating substrate binding | F93 | FasV | DOI: 10.1002/anie.202418843 |
| 57 | catalytic activity; dual activation mechanism | K76 | FasV | DOI: 10.1002/anie.202418843 |
| - | weak halogenation activity | L309A | FasV | DOI: 10.1002/anie.202418843 |