CylC

Enzyme Information

reference: 10.1038/nchembio.2421
host: E. coli
note: In the study, they couldn't obtain CylC alone, they ended up using N-terminally His6-tagged CylC when coexpressed with CylA and NHis6-CylB in E. coli BAP1. They also found that CylB and CylC probably form a complex.
producer: Cylindrospermum licheniforme ATCC 29412
phmm-model: dimetal-carboxylate.hmm

Gene Data

phmm	effect	mutation	gene_name	reference
-	comparable iron stoichometry relative to the WT	D282A	CylC	DOI: doi.10.1021/acs.biochem.4c00720
-	comparable iron stoichometry relative to the WT	E252A	CylC	DOI: doi.10.1021/acs.biochem.4c00720
-	active site	HXXDEXH	CylC	DOI: doi.10.1021/acs.biochem.4c00720
-	comparable iron stoichometry relative to the WT	Q128A	CylC	DOI: doi.10.1021/acs.biochem.4c00720
-	active site	QEXXH	CylC	DOI: doi.10.1021/acs.biochem.4c00720
283	putative active site residue	E283	CylC	DOI: doi.10.1021/acs.biochem.4c00720
-	H-bond acceptor	E252?	CylC	DOI: doi.10.1021/acs.biochem.4c00720
314	putative active site residue	E314	CylC	DOI: doi.10.1021/acs.biochem.4c00720
-	H-bond acceptor	D282	CylC	DOI: doi.10.1021/acs.biochem.4c00720
-	putative active site residue	H317	CylC	DOI: doi.10.1021/acs.biochem.4c00720
-	putative active site residue	Q135	CylC	DOI: doi.10.1021/acs.biochem.4c00720
310	putative active site residue	H310	CylC	DOI: doi.10.1021/acs.biochem.4c00720
313	putative active site residue	D313	CylC	DOI: doi.10.1021/acs.biochem.4c00720
-	putative active site residue	E103	CylC	DOI: doi.10.1021/acs.biochem.4c00720
-	putative active site residue	E136	CylC	DOI: doi.10.1021/acs.biochem.4c00720
-	putative active site residue	E139	CylC	DOI: doi.10.1021/acs.biochem.4c00720