CurA

Enzyme Information
reference: 10.1073/pnas.1006738107
host: E. coli
test: FTICR-MS and (IRMPD)-based PPant ejection assay
producer: Lyngbya majuscula
phmm-model: NHFe_variant_B.hmm

Gene Data
phmm effect mutation gene_name reference
- reduced Cl-HMG formation compared to WT K48A CurA DOI: 10.1073/pnas.1006738107
- reduced Cl-HMG formation compared to WT K50A CurA DOI: 10.1073/pnas.1006738107
- reduced Cl-HMG formation compared to WT Y68A CurA DOI: 10.1073/pnas.1006738107
- reduced Cl-HMG formation compared to WT Y68F CurA DOI: 10.1073/pnas.1006738107
- reduced Cl-HMG formation compared to WT R241A/R247E CurA DOI: 10.1073/pnas.1006738107
109 active site; stabilizes an extended conformation of alpha-KG F103 CurA DOI: 10.1073/pnas.1006738107
118 active site; contact alpha-KG, hydrophobic binding site L112 CurA DOI: 10.1073/pnas.1006738107
72 stabilize the structure D69 CurA DOI: 10.1073/pnas.1006738107
71 stabilize the structure Y68 CurA DOI: 10.1073/pnas.1006738107
125 active site; binding Cl S120 CurA DOI: 10.1073/pnas.1006738107
150 active site W134 CurA DOI: 10.1073/pnas.1006738107
148 active site S132 CurA DOI: 10.1073/pnas.1006738107
121 active site; binding Fe H115 CurA DOI: 10.1073/pnas.1006738107
- almost the same percentage of Cl-HMG formation compared to WT L112I CurA DOI: 10.1073/pnas.1006738107
- reduced Cl-HMG formation compared to WT S120A CurA DOI: 10.1073/pnas.1006738107
- reduced Cl-HMG formation compared to WT S132A CurA DOI: 10.1073/pnas.1006738107
- reduced Cl-HMG formation compared to WT R241A CurA DOI: 10.1073/pnas.1006738107
- reduced Cl-HMG formation compared to WT R247A CurA DOI: 10.1073/pnas.1006738107
- reduced Cl-HMG formation compared to WT S44A CurA DOI: 10.1073/pnas.1006738107
105 stabilize the structure H99 CurA DOI: 10.1073/pnas.1006738107
259 active site; binds alpha-KG R247 CurA DOI: 10.1073/pnas.1006738107
253 active site; binds alpha-KG; conserved among halogenases and oxygenases R241 CurA DOI: 10.1073/pnas.1006738107
- stabilize the structure D283 CurA DOI: 10.1073/pnas.1006738107
242 active site; binds alpha-KG S230 CurA DOI: 10.1073/pnas.1006738107
240 active site; binding Fe H228 CurA DOI: 10.1073/pnas.1006738107
257 active site; contact alpha-KG, hydrophobic binding site V245 CurA DOI: 10.1073/pnas.1006738107
234 active site; binding Cl; contact alpha-KG, hydrophobic binding site F222 CurA DOI: 10.1073/pnas.1006738107
163 active site; contact alpha-KG, hydrophobic binding site M148 CurA DOI: 10.1073/pnas.1006738107