CtNTH

Enzyme Information
reference: 10.1038/s41467-024-49147-7
host: E. coli BL21 (DE3)
test: 30 microM protein, 0.5 mM (NH)4Fe(SO4), 2.5 mM alpha-ketoglutaric acid sodium salt, 1 mM ascorbic acid, 10 mM NaBr/NaI/NaN3/NaNO2/NaOCN/NaSCN and 1 mM dGMP in HEPES buffer (pH 7.5)
producer: Catellatospora tritici
phmm-model: NHFe_nucleotide.hmm

Gene Data
phmm effect mutation gene_name reference
- substrate binding (H-bond with phosphate) R99 CtNTH DOI: 10.1038/s41467-024-49147-7
- substrate binding (H-bond with nucleobase) D106 CtNTH DOI: 10.1038/s41467-024-49147-7
- substrate binding (H-bond with phosphate) K108 CtNTH DOI: 10.1038/s41467-024-49147-7
- substrate binding (H-bond with phosphate) R178 CtNTH DOI: 10.1038/s41467-024-49147-7
130 substrate binding (H-bond with nucleobase) R306 CtNTH DOI: 10.1038/s41467-024-49147-7
95 phosphate-binding site V273(270) CtNTH DOI: 10.1038/s41467-024-49147-7
96 phosphate-binding site V274(271) CtNTH DOI: 10.1038/s41467-024-49147-7
20 substrate binding H195 CtNTH DOI: 10.1038/s41467-024-49147-7
128 phosphate-binding site G304 CtNTH DOI: 10.1038/s41467-024-49147-7
97 substrate binding F272 CtNTH DOI: 10.1038/s41467-024-49147-7
129 phosphate-binding site L305 CtNTH DOI: 10.1038/s41467-024-49147-7
63 phosphate-binding site R238 CtNTH DOI: 10.1038/s41467-024-49147-7
125 phosphate-binding site V301 CtNTH DOI: 10.1038/s41467-024-49147-7
78 substrate binding H253 CtNTH DOI: 10.1038/s41467-024-49147-7
- reduced activity compared to WT R99A CtNTH DOI: 10.1038/s41467-024-49147-7
- no activity K108A CtNTH DOI: 10.1038/s41467-024-49147-7
- no activity R178A CtNTH DOI: 10.1038/s41467-024-49147-7
- no activity D106A CtNTH DOI: 10.1038/s41467-024-49147-7
- slightly reduced activity compared to WT H193A CtNTH DOI: 10.1038/s41467-024-49147-7
- slightly reduced activity compared to WT R238A CtNTH DOI: 10.1038/s41467-024-49147-7
- reduced activity compared to WT R306A CtNTH DOI: 10.1038/s41467-024-49147-7
- highly reduced activity compared to WT F272A CtNTH DOI: 10.1038/s41467-024-49147-7
63 substrate binding (H-bond with nucleobase) R238 CtNTH DOI: 10.1038/s41467-024-49147-7
- phosphate-binding site I176 CtNTH DOI: 10.1038/s41467-024-49147-7