CmlS

Enzyme Information

reference: 10.1099/mic.0.26319-0
test: gene function disruption
producer: Streptomyces venezuelae ISP5230

reference: 10.1016/j.jmb.2010.01.020
test: crystallography and mutagenesis
producer: Streptomyces venezuelae
phmm-model: FDH_all_conventional.hmm

Gene Data

phmm	effect	mutation	gene_name	reference
-	different denaturation and dilufion in 6M GdHCl compared to WT	D277N	CmlS	DOI: 10.1016/j.jmb.2010.01.020
18	conserved for hydrogen bonding with the cofactor (says D33 in the paper)	E33	CmlS	DOI: 10.1016/j.jmb.2010.01.020
24	hydrogen bond with 3' hydroxyl of the adenosine moiety	R39	CmlS	DOI: 10.1016/j.jmb.2010.01.020
19	H-bond with the adenosine N7 and ribitol O3 hydroxyl	R35	CmlS	DOI: 10.1016/j.jmb.2010.01.020
286	H-bond with the adenosine N7 and ribitol O3 hydroxyl	D295	CmlS	DOI: 10.1016/j.jmb.2010.01.020
26	stabilize oxygens of the pyrophosphate moiety	R41	CmlS	DOI: 10.1016/j.jmb.2010.01.020
97	stabilize oxygens of the pyrophosphate moiety	R107	CmlS	DOI: 10.1016/j.jmb.2010.01.020
268	covalent linkage with the 8alpha carbon of the flavin ring	D277	CmlS	DOI: 10.1016/j.jmb.2010.01.020
269	juts from the solvent-exposed surface	W278	CmlS	DOI: 10.1016/j.jmb.2010.01.020
226	highly conserved in FDHs	R234	CmlS	DOI: 10.1016/j.jmb.2010.01.020