CiVCPO

Enzyme Information
reference: j b c . o r g / a r t i c l e / S 0 0 2 1 - 9 2 5 8 ( 1 9 ) 5 5 4 9 7 - 7 / f u l l t e x t
host: Saccharomyces cerevisiae (BJ1991)
test: MCD assay
producer: Curvularia inaequalis
phmm-model: VHPO_VCPO_nonselective.hmm
halide-source: ˝appropriate concentrations of the substrates chloride or bromide˝

Gene Data
phmm effect mutation gene_name reference
140 negative charge, residues poteintially involved in substrate-binding Glu196 CiVCPO DOI: 10.1021/acscatal.3c00761
392 positive charge-vanadate; HOX will target C3; H-bond; stabilize the pentacoordinated transition state; contact to two of the equatorial oxygens in VCPO Arg490 CiVCPO DOI: 10.1021/acscatal.3c00761 DOI: 10.1038/s41589-019-0355-x
269 HOX will target C3; stabilize the pentacoordinated transition state Lys353 CiVCPO DOI: 10.1021/acscatal.3c00761 DOI: S0021-9258(19)55497-7 DOI: 10.1074/jbc.275.16.11650
277 positive charge-vanadate; HOX will target C3; H-bond; stabilize the pentacoordinated transition state Arg360 CiVCPO DOI: 10.1021/acscatal.3c00761 DOI: S0021-9258(19)55497-7 DOI: 10.1074/jbc.275.16.11650
- bromoperoxidase; no chlorination K353A CiVCPO DOI: S DOI: 0 DOI: 0 DOI: 2 DOI: 1 DOI: - DOI: 9 DOI: 2 DOI: 5 DOI: 8 DOI: ( DOI: 1 DOI: 9 DOI: ) DOI: 5 DOI: 5 DOI: 4 DOI: 9 DOI: 7 DOI: - DOI: 7
- bromoperoxidase; no chlorination R360A CiVCPO DOI: S DOI: 0 DOI: 0 DOI: 2 DOI: 1 DOI: - DOI: 9 DOI: 2 DOI: 5 DOI: 8 DOI: ( DOI: 1 DOI: 9 DOI: ) DOI: 5 DOI: 5 DOI: 4 DOI: 9 DOI: 7 DOI: - DOI: 7
- bromoperoxidase; no chlorination R490A CiVCPO DOI: S DOI: 0 DOI: 0 DOI: 2 DOI: 1 DOI: - DOI: 9 DOI: 2 DOI: 5 DOI: 8 DOI: ( DOI: 1 DOI: 9 DOI: ) DOI: 5 DOI: 5 DOI: 4 DOI: 9 DOI: 7 DOI: - DOI: 7
- lower activity (Km for chloride strongly dependent of pH, Km for bromide not significantly dependent on pH); not crucial for halogenation activity S402A CiVCPO DOI: 10.1016/S0020-1693(03)00476-6
- no chlorination; bromination H404A CiVCPO DOI: 10.1074/jbc.275.16.11650
- reduction in chlorination R360A CiVCPO DOI: 10.1074/jbc.275.16.11650
- no chlorination R490A CiVCPO DOI: 10.1074/jbc.275.16.11650
- no chlorination; some peroxidase activity; decreased phosphatase activity K353A CiVCPO DOI: 10.1074/jbc.275.16.11650
- inactive at low pH, but is reversible at pH 8.3; pH 4 in the presence of H2O2 doesn't result in inactivation; chlorination seems to be pH dependent D292A CiVCPO DOI: 10.1074/jbc.275.16.11650
- partial loss of chloroperoxidase activity; higher affinity and rate of oxidation reaction; competition between this halide binding site and peroxide-lower concentrations of H2O2 lead to stronger inhibition by bromide; better bromoperoxidase at high pH F397H CiVCPO DOI: 10.1016/S0020-1693(03)00476-6
- cannot bind canadate covalently -> inactive enzyme H496A CiVCPO DOI: S DOI: 0 DOI: 0 DOI: 2 DOI: 1 DOI: - DOI: 9 DOI: 2 DOI: 5 DOI: 8 DOI: ( DOI: 1 DOI: 9 DOI: ) DOI: 5 DOI: 5 DOI: 4 DOI: 9 DOI: 7 DOI: - DOI: 7
315 positive charge-vanadate, may assist C-X bond formation Lys394 CiVCPO DOI: 10.1021/acscatal.3c00761
311 negative charge, may assist C-X bond formation 2-phenylindole/methylphenylindole substrate-enzyme complex Asp390 CiVCPO DOI: 10.1021/acscatal.3c00761
314 2-phenylindole/methylphenylindole substrate-enzyme complex Phe393 CiVCPO DOI: 10.1021/acscatal.3c00761
317 2-phenylindole/methylphenylindole substrate-enzyme complex Pro396 CiVCPO DOI: 10.1021/acscatal.3c00761
318 2-phenylindole/methylphenylindole substrate-enzyme complex Phe397 CiVCPO DOI: 10.1021/acscatal.3c00761
325 conserved in acid-phosphatases H404 CiVCPO DOI: 10.1074/jbc.275.16.11650
- 2-phenylindole/methylphenylindole substrate-enzyme complex Pro47 CiVCPO DOI: 10.1021/acscatal.3c00761
- 2-phenylindole/methylphenylindole substrate-enzyme complex His222 CiVCPO DOI: 10.1021/acscatal.3c00761
- 2-phenylindole/methylphenylindole substrate-enzyme complex Gln220 CiVCPO DOI: 10.1021/acscatal.3c00761
220 2-phenylindole/methylphenylindole substrate-enzyme complex Gly293 CiVCPO DOI: 10.1021/acscatal.3c00761
219 2-phenylindole/methylphenylindole substrate-enzyme complex; unlikely that it is a proton donor (phosphatase activity of D292A) Asp292 CiVCPO DOI: 10.1021/acscatal.3c00761 DOI: 10.1074/jbc.275.16.11650