CVBPO

Enzyme Information

reference: 10.1016/S0021-9258(19)89233-5
test: MCD assay and I2 formation for iodination testing
producer: Corallina pilulifera
halide-source: potassium fluoride, chloride, bromide, potassium iodide

reference: 10.1007/s00775-005-0639-3
note: Ca,Mg and strontium increases stability, vanadate increases thermostability
phmm: VHPO_VBPO.hmm
test: X-ray crystallography
producer: Corallina pilulifera

Gene Data

phmm	effect	mutation	gene_name	reference
-	no activity	R397Y	CVBPO	DOI: 10.1110/ps.03496004
323	None	R397	CVBPO
-	significant chloroperoxidase and bromoperoxidase activity; low incorporation rate of vanadate into the active site; might hinder the incorporation of vanadate	R397W	CVBPO	DOI: 10.1110/ps.03496004
-	significant chloroperoxidase and bromoperoxidase activity	R397F	CVBPO	DOI: 10.1110/ps.03496004
-	greater CPO activity	R397L	CVBPO	DOI: 10.1110/ps.03496004
-	greater CPO activity	R397Q	CVBPO	DOI: 10.1110/ps.03496004
-	greater CPO activity	R397E	CVBPO	DOI: 10.1110/ps.03496004
-	lost BPO activity (small side chain)	R397G	CVBPO	DOI: 10.1110/ps.03496004
-	lost BPO activity (small side chain)	R397A	CVBPO	DOI: 10.1110/ps.03496004
-	lost BPO activity (small side chain)	R397P	CVBPO	DOI: 10.1110/ps.03496004
-	lost BPO activity (small side chain)	R397S	CVBPO	DOI: 10.1110/ps.03496004