BrvH

Enzyme Information
reference: 10.1371/journal.pone.0196797
host: Escherichia coli pGro7 and BL21 (DE3)
test: mutagenesis, crystallization and activity assay
notes: enzymes (250 μl in 1 mL) with 1 μM FAD, 100 μM NAD, 100 mM NaBr/NaCl, PrnF (2.5 U/mL) and ADH (2 U/mL)
phmm-model: FDH_conventional.hmm
flavin-reductase: plasmid pClBhis-PrnF

Gene Data
phmm effect mutation gene_name reference
57 active site; directly accessible to solvent; 10.1002/cbic.202000444 K83 BrvH DOI: 10.1371/journal.pone.0196797; 10.1002/cbic.202000444
60 indole binding I86 BrvH DOI: 10.1002/cbic.202000444
32 indole binding I57 BrvH DOI: 10.1002/cbic.202000444
400 differs between BrvH and RebH M442 BrvH DOI: 10.1371/journal.pone.0196797
401 differs between BrvH and RebH F443 BrvH DOI: 10.1371/journal.pone.0196797
297 assumed halide binding site T351 BrvH DOI: 10.1371/journal.pone.0196797
298 assumed halide binding site; G360 in RebH S352 BrvH DOI: 10.1371/journal.pone.0196797
- substrate binding site; indole binding F102 BrvH DOI: 10.1371/journal.pone.0196797; 10.1002/cbic.202000444
- indole binding P101 BrvH DOI: 10.1002/cbic.202000444
295 substrate binding site; indole binding E349 BrvH DOI: 10.1371/journal.pone.0196797; 10.1002/cbic.202000444
- substrate binding site H100 BrvH DOI: 10.1371/journal.pone.0196797
None doesn't halogenate indole K83A BrvH DOI: 10.1371/journal.pone.0196797