BesD

Enzyme Information

reference: 10.1038/s41586-019-1020-y
test: knockout; L-lysine incubated with purified BesD, 4 mM HCl, NaCl, 5 mM sodium ascorbate, 1mM (NH4)2Fe(SO4)2*6H2O in 100 mM MOPS buffer and MS and NMR analysis
producer: Streptomyces cattleya
phmm-model: NHFe_small_amino_acids.hmm

Gene Data

phmm	effect	mutation	gene_name	reference
64	binding site; with the carboxylate group; relevant for being varian tA	R74	BesD	DOI: 10.1038/s41589-019-0355-x
-	binding site; with the carboxylate group; second sphere interaction	H134	BesD	DOI: 10.1038/s41589-019-0355-x
-	no hydroxylated or chlorinated compounds	R74A	BesD	DOI: 10.1038/s41589-019-0355-x
-	no hydroxylated or chlorinated compounds	D140A	BesD	DOI: 10.1038/s41589-019-0355-x
-	no hydroxylated or chlorinated compounds	E120A	BesD	DOI: 10.1038/s41589-019-0355-x
-	no hydroxylated or chlorinated compounds	W238A	BesD	DOI: 10.1038/s41589-019-0355-x
-	no hydroxylated or chlorinated compounds	W239A	BesD	DOI: 10.1038/s41589-019-0355-x
-	increased overall enzyme activity and abolished enzyme selectivity; decreased halogenation to hydroxylation ratios	H134A	BesD	DOI: 10.1038/s41589-019-0355-x
-	abolished selectivity for halogenation, reduced activity as compared to wild-type BesD	N219A	BesD	DOI: 10.1038/s41589-019-0355-x
-	no effect on activity or halogenation selectivity	T221A	BesD	DOI: 10.1038/s41589-019-0355-x
-	differs between different regioselectivities	Loop 117-119	BesD	DOI: 10.1038/s41589-019-0355-x
297	binding site; with the carboxylate group; epsilon-position preference	W238	BesD	DOI: 10.1038/s41589-019-0355-x
121	Fe coordination	H137	BesD	DOI: 10.1038/s41589-019-0355-x
240	Fe coordination	H204	BesD	DOI: 10.1038/s41589-019-0355-x
298	stacks over the aliphatic side chain of lysine, closes over the substrate at the entrance of the active site; epsilon-position preference	W239	BesD	DOI: 10.1038/s41589-019-0355-x
125	binding site; with the alpha-amine	Asp140	BesD	DOI: 10.1038/s41589-019-0355-x
107	binding site; with the epsilon-amine	E120	BesD	DOI: 10.1038/s41589-019-0355-x
257	binding site; with the epsilon-amine; second sphere interaction; highly conserved in halogenase homologs	N219	BesD	DOI: 10.1038/s41589-019-0355-x
259	water network with chloride, binding with epsilon-amine binding	T221	BesD	DOI: 10.1038/s41589-019-0355-x
-	has similar secondary structure to the WT	G139D	BesD	DOI: 10.1038/s41586-019-1020-y