AmVBPO1

Enzyme Information
reference: 10.5281/zenodo.14544279
host: E. coli (BL21)
test: UV MCD assay
producer: Acaryochloris marina MBIC 11017
halide-source: 200 mM KCl or KBr

reference: 10.1002/cbic.201600417
host: E. coli
test: X-ray crystallography
producer: Acaryochloris marina
phmm-model: VHPO_VBPO.hmm

Gene Data
effect mutation gene_name reference pHMM_position
vanadate H-bond His513 AmVBPO1 DOI: 10.1002/cbic.201600417
vanadate H-bond Asp598 AmVBPO1 DOI: 10.1002/cbic.201600417
vanadate H-bond His520 AmVBPO1 DOI: 10.1002/cbic.201600417
reacts well to small aa substitutions (predicted); small sidechains increase solvent accessibility at the enzymatic site; critical for substrate specificity and plays role in halogen specificity R425 AmVBPO1 DOI: 10.5281/zenodo.14544279
less stabilized in R425 compared to WT F401 AmVBPO1 DOI: 10.5281/zenodo.14544279
vanadate H-bond Asp363 AmVBPO1 DOI: 10.1002/cbic.201600417
intermolecular disulfide bonds between Cys of two neighbouring monomers Cys341 AmVBPO1 DOI: 10.1002/cbic.201600417
bond Cys341; tolerance to heat and solvents Cys206 AmVBPO1 DOI: 10.1002/cbic.201600417
bond with Cys341; tolerance to heat and solvents Cys308 AmVBPO1 DOI: 10.1002/cbic.201600417
increased bromination and chlorination of monochlorodimedone; prefers aryl derivatives; similar substrate scope to WT; doesn*t accept carbazoles or stirbenes R425S AmVBPO1 DOI: 10.5281/zenodo.14544279
no chlorination activity; lack of structured conformation of residues 400-405 R425D AmVBPO1 DOI: 10.5281/zenodo.14544279
TMB binding region, only structured in R425S; flexible in WT 390-404 AmVBPO1 DOI: 10.5281/zenodo.14544279
electrophilic aromatic chlorination His513 AmVBPO1 DOI: 10.5281/zenodo.14544279
no chlorination; slowed down for bromination R425S;F401G AmVBPO1 DOI: 10.5281/zenodo.14544279
decrease of chlorination rate of MCD and phenol; chlorination of TMB to low extent R425;E139G AmVBPO1 DOI: 10.5281/zenodo.14544279
favors the anchoring of aromatic substrates Glu139 AmVBPO1 DOI: 10.5281/zenodo.14544279
favors the anchoring of aromatic substrates Glu399 AmVBPO1 DOI: 10.5281/zenodo.14544279
vanadate H-bond; active site Lys428 AmVBPO1 DOI: 10.1002/cbic.201600417; 10.5281/zenodo.14544279