AetF

Enzyme Information

reference: 10.1021/jacs.1c12778
host: Escherichia coli BL21(DE3)
notes: single component, binds FAD confirmed by LC-MS, preference for NADPH over NADH
producer: Aetokthonos hydrillicola
phmm-model: FDH_unconventaional.hmm
halide-source: 10 mM KBr

Gene Data

phmm	effect	mutation	gene_name	reference
257	catalytic residue? might have similar function as Lys277 in bmp5	Lys258	AetF	DOI: 10.1107/S2059798323004254
216	H-bond between its carbonyl group and the NH group of indole	Met216	AetF	DOI: 10.1107/S2059798323004254
199	side chain O forms H-bond with the amino group of Trp	Leu199	AetF	DOI: 10.1107/S2059798323004254
215	side chain O forms H-bond with the amino group of Trp	Leu215	AetF	DOI: 10.1107/S2059798323004254
585	salt bridge with Trp carboxylate; water molecule links its side chain with the Trp carboxylate	Lys587	AetF	DOI: 10.1107/S2059798323004254
522	side chain forms H-bond with the carboxylate of Trp	Ser523	AetF	DOI: 10.1107/S2059798323004254
515	water molecule links its side chain with the Trp carboxylate	Asp516	AetF	DOI: 10.1107/S2059798323004254
200	indole binding site; surrounds the indole moiety of 5-Br-Trp	Glu200	AetF	DOI: 10.1107/S2059798323004254
499	side chain O forms H-bond with the amino group of Trp; side chain forms H-bond with the carboxylate of Trp	Gln500	AetF	DOI: 10.1107/S2059798323004254
183	surrounds the indole moiety of 5-Br-Trp	Leu183	AetF	DOI: 10.1107/S2059798323004254
196	surrounds the indole moiety of 5-Br-Trp and forms a H-bond with its carbonyl group to the NH group	Leu196	AetF	DOI: 10.1107/S2059798323004254
216	surrounds the indole moiety of 5-Br-Trp	Met216	AetF	DOI: 10.1107/S2059798323004254
219	surrounds the indole moiety of 5-Br-Trp	Pro219	AetF	DOI: 10.1107/S2059798323004254
523	surrounds the indole moiety of 5-Br-Trp	Phe524	AetF	DOI: 10.1107/S2059798323004254
372	indole binding site; surrounds the indole moiety of 5-Br-Trp	Thr373	AetF	DOI: 10.1107/S2059798323004254